Journal: Neural Regeneration Research

Article Title: Müller cells are activated in response to retinal outer nuclear layer degeneration in rats subjected to simulated weightlessness conditions

doi: 10.4103/NRR.NRR-D-23-01035

Figure Lengend Snippet: Simulated weightlessness can lead to Müller cell gelatinization. (A) Representative images of GFAP immunofluorescence (green, stained with Alexa Fluor TM 488). (B) Quantification of GFAP-immunopositive cells. There were more GFAP-immunopositive cells in the TS group than in the CON group. (C) Representative immunofluorescence images of GS (red, stained with Cy3). Scale bar: 50 µm. (D) Quantification of GS-immunopositive cells. There were more GS-immunopositive cells in the CON group than in the TS group. (E) Western blot of GFAP, GS, and GLAST. (F–H) Quantification of GFAP, GS, and GLAST relative protein expression. Data are expressed as mean ± SD ( n = 4 per group). * P < 0.05, ** P < 0.01 (independent samples t -test). CON: Control; DAPI: 4′,6-diamidino-2-phenylindole; GAPDH: glyceraldehyde-3-photosphate dehydrogenase; GCL: ganglion cell layer; GFAP: glial fibrous acidic protein; GLAST: L-glutamate/L-aspartate transporter; GS: glutamine synthetase; INL: inner nuclear layer; ONL: outer nuclear layer; TS: tail suspension.

Article Snippet: The tissue sections were then incubated overnight at 4°C with primary antibodies targeting sex determining region Y-box 2 (SOX2; mouse, Proteintech, Wuhan, China, Cat# 66411-1-Ig, RRID: AB_2881783), paired box gene 6 (PAX6; mouse; Proteintech; Cat# 12323-1-AP, RRID: AB_2159695), cellular retinaldehyde-binding protein (CRALBP; rabbit, Proteintech, Cat# 67529-1-Ig, RRID: AB_2882748), glial fibrous acidic protein (GFAP; rabbit, Proteintech, Cat# 16825-1-AP, RRID: AB_2109646), glutamine synthetase (GS; rabbit, Proteintech, Cat# 66323-2-Ig, RRID: AB_2881704), cone-rod homeobox (crx; rabbit, Proteintech, Cat# 12047-1-AP, RRID: AB_2292128), and rhodopsin (mouse, bs-19872R; Bioss, Beijing, China, Cat# 129-10588, RRID: AB_10565214) at 1:200 dilutions.

Techniques: Immunofluorescence, Staining, Western Blot, Expressing, Control, Suspension

Journal: Neural Regeneration Research

Article Title: Müller cells are activated in response to retinal outer nuclear layer degeneration in rats subjected to simulated weightlessness conditions

doi: 10.4103/NRR.NRR-D-23-01035

Figure Lengend Snippet: Effect of simulated weightlessness on Müller cell neural differentiation. Immunofluorescence double staining of the rat retina. Red indicates the retinal Muller cell marker CRALBP (Cy3), green indicates the retinal progenitor cell marker Crx or rhodopsin (Alexa Fluor TM 488), and blue indicates nuclear DAPI staining. (A) Immunofluorescence images of Crx staining. (B) Quantification of Crx-immunopositive cells. There were more Crx-immunopositive cells in the TS group than in the CON group. (C) Rhodopsin immunofluorescence images. Scale bar: 20 µm (left four columns), 50 µm (rightmost column). (D) Quantification of rhodopsin-immunopositive cells. There were more rhodopsin-immunopositive cells in the TS4W group than in the CON4W group. Data are expressed as mean ± SD ( n = 4 per group). ** P < 0.01 (independent samples t -test). CON: Control; Crx: cone-rod homeobox; DAPI: 4′,6-diamidino-2-phenylindole; CRALBP: cellular retinaldehyde-binding protein; TS4W: tail suspension for 4 weeks; TS8W: tail suspension for 8 weeks.

Article Snippet: The tissue sections were then incubated overnight at 4°C with primary antibodies targeting sex determining region Y-box 2 (SOX2; mouse, Proteintech, Wuhan, China, Cat# 66411-1-Ig, RRID: AB_2881783), paired box gene 6 (PAX6; mouse; Proteintech; Cat# 12323-1-AP, RRID: AB_2159695), cellular retinaldehyde-binding protein (CRALBP; rabbit, Proteintech, Cat# 67529-1-Ig, RRID: AB_2882748), glial fibrous acidic protein (GFAP; rabbit, Proteintech, Cat# 16825-1-AP, RRID: AB_2109646), glutamine synthetase (GS; rabbit, Proteintech, Cat# 66323-2-Ig, RRID: AB_2881704), cone-rod homeobox (crx; rabbit, Proteintech, Cat# 12047-1-AP, RRID: AB_2292128), and rhodopsin (mouse, bs-19872R; Bioss, Beijing, China, Cat# 129-10588, RRID: AB_10565214) at 1:200 dilutions.

Techniques: Immunofluorescence, Double Staining, Marker, Staining, Control, Binding Assay, Suspension

Journal: iScience

Article Title: Pharmaceutical inhibition of the Chk2 kinase mitigates cone photoreceptor degeneration in an iPSC model of Bardet-Biedl syndrome

doi: 10.1016/j.isci.2025.112130

Figure Lengend Snippet:

Article Snippet: Rabbit anti-CORD2 (CRX) , GenTex , GTX124188.

Techniques: Plasmid Preparation, Recombinant, Full Display Name, TUNEL Assay, Imaging, Ab Array, Generated, Software, Microscopy, Extraction